Lab Publications

  1. Camire, R.M., Kalafatis, M., Simioni, P., Girolami, A., and Tracy, P.B. Platelet-derived factor Va and factor VaLeiden cofactor activities are sustained on the surface of activated platelets despite the presence of activated protein C. Blood. 91, 2818-2829, 1998.
  2. Larson, P.J., Camire, R.M., Wong, D., Fasano, N.C., Monroe, D.M., Tracy, P.B., and High, K.A. Structure/function analyses of recombinant variants of human factor Xa: Factor Xa incorporation into prothrombinase on the activated platelet surface is not mimicked by synthetic phospholipid vesicles. Biochemistry. 37, 5029-5038, 1998.
  3. Camire, R.M., Kalafatis, M., and Tracy, P.B. Proteolysis of factor V by cathepsin G and elastase indicates that cleavage at Arg1545 optimizes cofactor function by facilitating factor Xa binding. Biochemistry. 37, 11896-11906, 1998.
  4. Camire, R.M., Pollak, E.S., Kaushansky, K., and Tracy, P.B. Secretable human platelet-derived factor V originates from the plasma pool. Blood. (Rapid Communication) 92, 3035-3041, 1998.
  5. Colman, R.W., Tracy, P.B., Camire, R.M., Pollak, E.S., and Kaushansky, K. Where does platelet factor V originate? Blood. 93, 3152-3154, 1999.
  6. Camire, R.M., Larson, P.J., Stafford D.W., and High, K.A. Enhanced γ-carboxylation of recombinant factor X using a chimeric construct containing the prothrombin propeptide. Biochemistry. 39, 14322-14329, 2000.
  7. Arruda, V.R., Hagstrom, J.N., Deitch, J., Patterson-Heiman, T., Camire, R.M., Chu, K., Fields, P.A., Herzog, R.W., Couto, L.B., Larson, P.J., and High, K.A. Posttranslational modifications of recombinant myotube-synthesized human factor IX. Blood. 97, 130-138, 2001.
  8. Camire, R.M. Prothrombinase assembly and S1 site occupation restores the catalytic activity of FXa impaired by mutation at the sodium-binding site. J. Biol. Chem. 277, 37863-37870, 2002.
  9. Toso, R., Tidd, T., Pinotti, M., Camire, R.M., Marchetti, G., High, K.A., Bernardi, F., and Pollak, E.S. Factor VII V17G models a zymogen-like form of factor VIIa. Biochem. J. 369, 563-571, 2003.
  10. Pinotti, M., Camire, R.M., Baroni, M., Marchetti, G., Rajab, A., and Bernardi, F. Impaired prothrombinase activity of Factor X Gly381Asp results in severe familial CRM+ FX deficiency. Thromb. Haemost. 89, 243-248, 2003
  11. Camire, R., Denchy, R.A., Day, G.A., Lanzano, S., Sheth, S., and Brown, S. Prenatal diagnosis of Factor X deficiency using a combination of direct mutation detection and linkage analysis with an intragenic single nucleotide polymorphism. Prenat. Diagn. 23, 457-460, 2003.
  12. Yarovoi, H., Kufrin, D., Eslin, D.E., Thornton, M.A., Haberickter, S.L., Zhu, H., Camire, R.M., Fakharzadeh, S.S., Kowalska, A., Wilcox, D.A., Montgomery, R.R., and Poncz, M. Factor VIII ectopically expressed in platelets: Efficacy in hemophilia A treatment. Blood. 102, 4006-4016, 2003.
  13. Margaritis, P., Arruda, V.R., Aljamali, M., Camire, R.M., Schlachterman, A., and High, K.A. Novel therapeutic approach for hemophilia using gene delivery of an engineered secreted activated factor VII. J. Clin. Invest. 113, 1025-1031, 2004.
  14. Toso, R. and Camire, R.M. Removal of B-domain sequences from factor V rather than specific proteolysis underlies the mechanism by which cofactor function is realized. J. Biol. Chem. 279, 21643-21650, 2004.
  15. Sun, Y.M., Jin, D.Y., Camire, R.M. and Stafford, D.W. Vitamin K epoxide reductase significantly improves carboxylation in a cell line over-expressing factor X. Blood 106, 3811-3815, 2005.
  16. Schlachterman, A., Schuettrumpf, J., Liu, J., Furlan Freguia, C., Toso, R., Poncz, M., Camire, R.M., and Arruda, V.R. Factor V Leiden improves hemostasis in murine hemophilia models. J. Thromb. Haemost. 3, 2730-2737, 2005.
  17. Pollak, E.S., Russell, T.T., Ptashkin, B., Smith-Whitley, K., Camire, R.M., and Bauer, K.A. Asymptomatic factor VII deficiency in African Americans. Am. J. Clin. Pathol. 126, 1-5, 2006.
  18. Toso, R. and Camire, R.M. Role of hirudin-like factor Va heavy chain sequences in prothrombinase function. J. Biol. Chem. 281, 8773-8779, 2006.
  19. Zhu, H., Toso, R. and Camire, R.M. Inhibitory sequences within the B-domain stabilize circulating factor V in an inactive state. J. Biol. Chem. 282, 15033-15039, 2007.
  20. Chen, L., Zhu, F., Li, J., Lu, H., Jiang, H., Sarkar, R., Arruda, V.R., Wang, J., Zhao, J., Pierce, G.F., Ding, Q., Wang, X., Wang, H., Pipe, S.W., Liu, X.Q., Xiao, X., Camire, R.M., Xiao, W. The enhancing effects of the light chain on heavy chain secretion in split delivery of factor VIII gene. Mol. Ther. 15, 1856-1862, 2007.
  21. Hacisalihoglu, A., Panizzi, P., Bock, P.E., Camire, R.M., and Krishnaswamy, S. Restricted active site docking by enzyme-bound substrate enforces the ordered cleavage of prothrombin by prothrombinase. J. Biol. Chem. 282, 32974-32982, 2007.
  22. Aljamali, M.N., Margaritis, P., Schlachterman, A., Tai, S.J., Roy, E., Bunte, R., Camire, R.M., and High, K.A. Long-term expression of murine factor VIIa is safe but elevated levels result in premature mortality. J. Clin. Invest. 118, 1825-1834, 2008.
  23. Cao, W., Krishnaswamy, S., Camire, R.M., Lenting, P.J., and Zheng, X.L. Factor VIII accelerates proteolytic cleavage of von Willebrand Factor by ADAMTS13. Proc. Natl. Acad. Sci. U.S.A. 105, 7416-7421, 2008.
  24. Toso, R., Zhu, H., and Camire, R.M. The conformational switch from the factor X zymogen to protease state mediates exosite expression and prothrombinase assembly. J. Biol. Chem. 283, 18627-18635, 2008.
  25. Jeimy, S.B., Fuller, N., Tasneem, S., Segers, K., Stafford, A.R., Weitz, J.I., Camire, R.M., Nicolaes, G.A.F., and Hayward, C.P.M. Multimerin 1 binds factor V and activated factor V with high affinity and inhibits thrombin generation. Thromb. Haemost. 100/6, 1058-1067, 2008.
  26. Bos, M.H.A., Boltz, M., St. Pierre, L., de Jersey, J., Masci, P.P., Lavin, M.F. and Camire, R.M. Venom factor V from the common brown snake escapes hemostatic regulation through procoagulant adaptations. In Press, Blood.